germany cat  (Qiagen)

Journal: BMC Cancer

Article Title: MiR-200c restoration inhibits FOXP3 and metastatic spread in breast cancer: evidence from in vitro and in vivo models

doi: 10.1186/s12885-026-15574-6

Figure Lengend Snippet: Restoration of miR200c inhibits non-metastatic and metastatic breast cancer cell proliferation and migration. a Effect of miR-200c modulation on viability of breast cancer cells, ( b ) Light microscope images of migrated cells, “cell migration assay,” stained with crystal violet, showed a significant reduction in the migration in BC cells transfected with miR-200c mimic. However, a significant increase in migrated cells was detected in BC cells after transfection with miR-200c inhibitor. c Effects of transfection of BC cells with miR-200c mimic and inhibitor on cell invasion measured by Boyden chamber invasion assay. The sample is performed in triplicate to ensure the accuracy of the test. Values represent the mean of three experiments ± Standard deviation, **, p-value < 0.01, *, p-value < 0.05. Comparative analysis was performed by One-Way ANOVA, followed by Tukey’s multicomparative test. Abbreviations: BC: breast cancer

Article Snippet: Next, 0.75 μL of HI Perfect Transfection Reagent , cat no: 301,704 , Qiagen , Hilden , Germany , is added to 24.25 μL of RPMI culture media without serum, and after 10 min incubation at 15–20 °C, 25 μL of the complex is added to each well, followed by the addition of 175 μL of DMEM media.

Techniques: Migration, Light Microscopy, Cell Migration Assay, Staining, Transfection, Invasion Assay, Standard Deviation

Journal: BMC Cancer

Article Title: MiR-200c restoration inhibits FOXP3 and metastatic spread in breast cancer: evidence from in vitro and in vivo models

doi: 10.1186/s12885-026-15574-6

Figure Lengend Snippet: Effect of transfection with miR-200c mimic on apoptosis of MDA-MB361 and MCF7 cell lines ( a ) Images of apoptosis assay by Annexin V/propidium iodide following transfection of miR-200c mimic, measured by Flowcytometry. b Percent of viable, early apoptotic, and necrotic cells. Values represent the mean of three experiments ± Standard deviation of three independent measurements for each group. * indicates significant difference between compared groups ( p < 0.05).Multiple group cmparisions was performed by One-Way ANOVA, followed by Tukey’s multicomparative test. Abbreviations: PI: propidium iodide, BC: breast cancer, PI: Propidium iodide

Article Snippet: Next, 0.75 μL of HI Perfect Transfection Reagent , cat no: 301,704 , Qiagen , Hilden , Germany , is added to 24.25 μL of RPMI culture media without serum, and after 10 min incubation at 15–20 °C, 25 μL of the complex is added to each well, followed by the addition of 175 μL of DMEM media.

Techniques: Transfection, Apoptosis Assay, Standard Deviation

Journal: BMC Cancer

Article Title: MiR-200c restoration inhibits FOXP3 and metastatic spread in breast cancer: evidence from in vitro and in vivo models

doi: 10.1186/s12885-026-15574-6

Figure Lengend Snippet: Effect of transfection with miR-200c mimic on the expression of miR-200c-5p and its target gene “mRNA-FOXP3”, measured by Syber green-based Real-time PCR. Values represent the mean of three experiments ± Standard deviation in three tested BC cell lines. a and b : miR-200c-5p and mRNA-FOXP3 expression (FC) after transfection with miR-200c mimic or inhibitor, compared to untreated cells. Values represent the mean of three independent measurements for each experiment ± Standard deviation. *: denotes a significant difference between the compared groups ( p < 0.05). Statistical significance was determined using a one-way ANOVA followed by Tukey’s post-hoc test. c Pearson’s correlation between the expression of miR-200c and FOXP3 in different groups. Abbreviations: BC: breast cancer, FC: fold change, r: correlation coefficient, CI: confidence interval

Article Snippet: Next, 0.75 μL of HI Perfect Transfection Reagent , cat no: 301,704 , Qiagen , Hilden , Germany , is added to 24.25 μL of RPMI culture media without serum, and after 10 min incubation at 15–20 °C, 25 μL of the complex is added to each well, followed by the addition of 175 μL of DMEM media.

Techniques: Transfection, Expressing, Real-time Polymerase Chain Reaction, Standard Deviation